跨膜電阻儀(新型EVOM MANUAL)
跨膜電阻儀(新型EVOM MANUAL)
Epithelial Volt/Ohm (TEER) Meter 3
型號:EVM-MT-03-01
搭配可替換刀片STX4電極
※應用特色※
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消除錯誤並減少實驗處理時間
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自動數據記錄,無需手動記錄數據
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數據以USB方式保存,並且可以CSV格式導出,以便在電子表格和其他數據分析程序中輕鬆使用
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佔地面積小,可提供更多的工作台空間
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易於校準和驗證,自動20倍採樣平均值和低噪聲設計
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腳踏板可免用手紀錄數據-腳踏開關還可以最大程度地減少細胞培養樣品中可能引入的污染物
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電池電量低時通過自動保存和數據恢復來防止數據丟失
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通過將單位面積公式應用於電阻可以輕鬆計算TEER
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具空白處理(Blank Handling)功能,可以自動刪除背景電壓或電位差值
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若採樣電阻值低於200歐姆,則EVOM3的測量分辨率為歐姆解析度的1/10
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新的STX4電極更加精確。為增重自立式電極可垂直位於孔板的頂部,因此測量時無需握住電極
- STX4電極具屏蔽電極可最大程度地減少電器干擾並更易於維護,可替換式刀片式電極。
※應用領域※
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測量上皮或內皮組織的融合,TEER和電位差
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細胞毒性
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滲透性,電導和藥物研究
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血腦屏障運輸(Blood–Brain Barrier,BBB)
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肺上皮組織研究、肺病毒感染
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腸道組織研究
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皮膚研究
- 腸道、腎臟和肝臟組織,如具有 Caco2 3D 組織功能的腸道藥物吸收研究
- COVID 研究的肺體外模型
包含以下組件(數量各1件):
(1) EVOM3 Meter
(2) STX4電極組
(3) 32G USB驅動器(300749)
(4) USB電纜(503535)
(5) 校準套件-1000Ω測試電阻 (99673)
(6) 電源線與充電(803025)
(7) 腳踏開關(13142)
可擴充配件:
(1) Endohm Cell Culture Cup Chambers
(2) STX100C96或STX100M-可適用於96孔盤電極
(3) STX3取代電極-可自由調節最大10mm間距
中文簡易操作手冊下載
※參考文獻※
Blood Brain Barrier
Pokharel, S., Gliyazova, N., Dandepally, S., Williams, A., & Ibeanu, G. (2022). Neuroprotective effects of an in vitro BBB permeable phenoxythiophene sulfonamide small molecule in glutamate-induced oxidative injury. Experimental and Therapeutic Medicine, 23(1). https://doi.org/10.3892/ETM.2021.11002
Elbakary, B., & Badhan, R. K. S. (2020). A dynamic perfusion based blood-brain barrier model for cytotoxicity testing and drug permeation. Scientific Reports 2020 10:1, 10(1), 1–12. https://doi.org/10.1038/s41598-020-60689-w
Neal, E. H., Marinelli, N. A., Shi, Y., McClatchey, P. M., Balotin, K. M., Gullett, D. R., … Lippmann, E. S. (2019). A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs. Stem Cell Reports, 12(6), 1380–1388. https://doi.org/10.1016/J.STEMCR.2019.05.008
Maherally, Z., Fillmore, H. L., Tan, S. L., Tan, S. F., Jassam, S. A., Quack, F. I., … Pilkington, G. J. (2018). Real‐time acquisition of transendothelial electrical resistance in an all‐human, in vitro , 3‐dimensional, blood‐brain barrier model exemplifies tight‐junction integrity. The FASEB Journal, 32(1), 168–182. https://doi.org/10.1096/fj.201700162R
COVID Studies
Robinot, R., Hubert, M., de Melo, G. D., Lazarini, F., Bruel, T., Smith, N., … Chakrabarti, L. A. (2021). SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance. Nature Communications 2021 12:1, 12(1), 1–16. https://doi.org/10.1038/s41467-021-24521-x
Samelson, A. J., Tran, Q. D., Robinot, R., Carrau, L., Rezelj, V. V., Kain, A. Mac, … Kampmann, M. (2021). BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2. BioRxiv. https://doi.org/10.1101/2021.01.19.427194
Shaban, M. S., Müller, C., Mayr-Buro, C., Weiser, H., Meier-Soelch, J., Albert, B. V., … Kracht, M. (2021). Multi-level inhibition of coronavirus replication by chemical ER stress. Nature Communications 2021 12:1, 12(1), 1–20. https://doi.org/10.1038/s41467-021-25551-1
Drug Discovery
Wu, X., Yin, C., Ma, J., Chai, S., Zhang, C., Yao, S., … Lin, G. (2021). Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo. Acta Pharmaceutica Sinica. B, 11(7), 1885–1902. https://doi.org/10.1016/J.APSB.2020.12.021
Epithelial/Endothelial Barrier Studies
Pongkorpsakol, P., Turner, J. R., & Zuo, L. (2020). Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity. Current Protocols in Immunology, 131(1). https://doi.org/10.1002/cpim.112
Haeger, J. D., Loch, C., & Pfarrer, C. (2018). The newly established bovine endometrial gland cell line (BEGC) forms gland acini in vitro and is only IFNτ-responsive (MAPK42/44 activation) after E 2 and P 4-pre-incubation. Placenta, 67, 61–69. https://doi.org/10.1016/J.PLACENTA.2018.05.009
Pham, V. T., Seifert, N., Richard, N., Raederstorff, D., Steinert, R., Prudence, K., & Mohajeri, M. H. (2018). The effects of fermentation products of prebiotic fibres on gut barrier and immune functions in vitro. PeerJ, 6, e5288. https://doi.org/10.7717/peerj.5288